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trypletm select 10x  (Thermo Fisher)


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    Structured Review

    Thermo Fisher trypletm select 10x
    Trypletm Select 10x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypletm+select+10x/PBS/pm41318626-86-37-40
    Average 99 stars, based on 1 article reviews
    trypletm select 10x - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Passaging:

    Article Title: Learning time-varying information flow from single-cell epithelial to mesenchymal transition data
    Article Snippet: Cells were cultured at 37°C in DMEM (D5671, Sigma Aldrich), supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin, at 5% CO 2. .. For cell passaging, cells were incubated with TrypLETM Select 10X (Life Technologies) in PBS in a 1:5 ratio (v/v) for 10 minutes at 37°C. ..

    Article Title: Learning Edge Rewiring in EMT From Single Cell Data
    Article Snippet: Cells were cultured at 37 °C in DMEM (D5671, Sigma Aldrich), supplemented with 10% FBS, 2 mM Lglutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin, at 5% CO 2. .. For cell passaging, cells were incubated with TrypLETM Select 10X (Life Technologies) in PBS in a 1:5 ratio (v/v) for 10 minutes at 37°C. ..

    Incubation:

    Article Title: Learning time-varying information flow from single-cell epithelial to mesenchymal transition data
    Article Snippet: Cells were cultured at 37°C in DMEM (D5671, Sigma Aldrich), supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin, at 5% CO 2. .. For cell passaging, cells were incubated with TrypLETM Select 10X (Life Technologies) in PBS in a 1:5 ratio (v/v) for 10 minutes at 37°C. ..

    Article Title: Learning Edge Rewiring in EMT From Single Cell Data
    Article Snippet: .. For cell harvest, cells were washed two times with PBS and incubated with TrypLETM Select 10X (Life Technologies) in PBS at a 1:5 ratio (v/v) for 10 minutes at 37°C. ..

    Article Title: Learning Edge Rewiring in EMT From Single Cell Data
    Article Snippet: Cells were cultured at 37 °C in DMEM (D5671, Sigma Aldrich), supplemented with 10% FBS, 2 mM Lglutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin, at 5% CO 2. .. For cell passaging, cells were incubated with TrypLETM Select 10X (Life Technologies) in PBS in a 1:5 ratio (v/v) for 10 minutes at 37°C. ..

    Article Title: Control of replication stress and mitosis in colorectal cancer stem cells through the interplay of PARP1, MRE11 and RAD51.
    Article Snippet: CRC-SCs were routinely maintained in Dulbecco’s Modified Eagle Medium (DMEM)/F12-based (#52100047, #21700018) culture medium specific for CSCs containing 4 mg/mL bovine serum albumin (BSA; #A1312, US Biological, Salem, MA), 1X Penicillin-Streptomycin-Amphotericin B (PSF; #17-745E, Lonza, Basel, Switzerland), 0.13% NaHCO3 (#25080094, Thermo Scientific), 6 mM Hepes (#15630056, Thermo Scientific), 2mM L-glutamine (#25030024, Thermo Scientific), 0.1 mg/mL apotransferrin (#T2252), 0.4 units heparin sodium salt (#H3393), 1.1% glucose (#G8769), 25 μg/mL insulin (#91077 C), 6.3 ng/mL progesterone (#P8783), 9.7 μg/mL putrescine dihydrochloride (#P5780), and 5.2 ng/mL sodium selenite (#S5261) and supplemented with 20 ng/mL human epidermal growth factor (EGF; #AF-100-15, PeproTech, London, UK), 10 ng/mL human basic fibroblast growth factor (bFGF; #AF-100-18B, PeproTech), and 10 mM nicotinamide (#N0636). .. All CRC-SCs including neoresistant (neoR) CRC-SCs (whose generation is described in the next session) were passaged once/twice a week at dilution 1:2 by mechanical dissociation, through a micropipette, and/or enzymatic dissociation, by incubating them for less than 5 min at 37 °C with TrypLETM Select 10X (#A1217701, Thermo Scientific) and Accumax (#A7089) (1:1, V:V), and incubated in standard culture conditions in ultra-low attachment tissue culture flasks. ..

    Article Title: Learning time-varying information flow from single-cell epithelial to mesenchymal transition data
    Article Snippet: .. For cell harvest, cells were washed two times with PBS and incubated with TrypLETM Select 10X (Life Technologies) in PBS at a 1:5 ratio (v/v) for 10 minutes at 37°C. ..

    Saline:

    Article Title: Differential effects of fetal bovine serum and human platelet lysate on mesenchymal stromal cell-mediated support of hematopoietic stem/progenitor cells: a functional and transcriptomic analysis.
    Article Snippet: .. Upon reaching 80-90% 203 confluence, MSC(M) were detached using either a solution of 0.05% (v/v) 204 Trypsin (Gibco) supplemented with 1 mM ethylenediaminetetraacetic acid 205 (EDTA) (Thermo Fisher Scientific) in phosphate-buffered saline (PBS) (Gibco) 206 or with TrypLETM Select 10x (Gibco) diluted 1:10 in PBS (a xeno-free alternative 207 for cell culture using DMEM-hPL). .. During each passage, cell number was 208 determined using the Trypan Blue (Gibco) exclusion method.

    Cell Culture:

    Article Title: Differential effects of fetal bovine serum and human platelet lysate on mesenchymal stromal cell-mediated support of hematopoietic stem/progenitor cells: a functional and transcriptomic analysis.
    Article Snippet: .. Upon reaching 80-90% 203 confluence, MSC(M) were detached using either a solution of 0.05% (v/v) 204 Trypsin (Gibco) supplemented with 1 mM ethylenediaminetetraacetic acid 205 (EDTA) (Thermo Fisher Scientific) in phosphate-buffered saline (PBS) (Gibco) 206 or with TrypLETM Select 10x (Gibco) diluted 1:10 in PBS (a xeno-free alternative 207 for cell culture using DMEM-hPL). .. During each passage, cell number was 208 determined using the Trypan Blue (Gibco) exclusion method.

    Article Title: Ex-vivo Expansion of Bone Marrow-derived Mesenchymal Stromal Cells for Clinical Use: the Starting Platelet Concentration of Human Platelet Lysate Affects Cell Proliferation, Senescence and Phenotype
    Article Snippet: .. Cells were detached at 85% con uence by adding TrypLETM Select 10X (Thermo sher Scienti c), microscopically counted with Bürker’s chamber and plated until passage 5 (p5) at 5.000 cells/cm2 in cell culture asks (Corning ® Rectangular Canted Neck Cell Culture Flask with Vent Cap). ..



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